Part:BBa_K1318000:Design

CcdB toxin

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 216
  

Design Notes

CcdB expression is toxic to bacteria (verified in E.coli). Express only using an inducible promoter or alongside its antidote ccdA.

Source

E.coli fertility factor (F plasmid), ccdBA operon.

References

• Miki, T., Ae Park, J., Nagao, K., Murayama, N., & Horiuchi, T. (1992). Control of segregation of chromosomal DNA by sex factor F in Escherichia coli: Mutants of DNA gyrase subunit A suppress letD (ccdB) product growth inhibition. Journal of molecular biology, 225(1), 39-52.

• Van Melderen, L. (2010). Toxin–antitoxin systems: why so many, what for? Current opinion in microbiology, 13(6), 781-785.